Mapping Dmef2-binding regulatory modules by using a ChIP-enriched in silico targets approach

• DOI: 10.1073/pnas.0507030102
• Publication Date: 2005-12-10T01:43:46Z
• Authors: Guillaume Junion; Teresa Jagla; Sebastien Duplant; Romain Tapin; Jean-Philippe Da ...; Krzysztof Jagla
• Keywords: Chromatin immunoprecipitation; Mef2; Immunoprecipitation

Mapping the regulatory modules to which transcription factors bind in vivo is a key step toward understanding of global gene expression programs. We have developed chromatin immunoprecipitation (ChIP)-chip strategy for identifying factor-specific regions acting vivo. This method, called ChIP-enriched silico targets (ChEST) approach, combines cross-linked protein-DNA complexes (X-ChIP) with prediction and generation computed DNA microarrays. report use ChEST Drosophila identify several previously unknown myocyte enhancer factor 2 (MEF2), regulator myogenic differentiation. Our approach was validated by demonstrating that identified sequences act as enhancers are able drive reporter specifically MEF2-positive muscle cells. Presented here, originally designed Drosophila, but it can be adapted any sequenced annotated genome.