Global identification of noncoding RNAs in Saccharomyces cerevisiae by modulating an essential RNA processing pathway

• DOI: 10.1073/pnas.0507669103
• Publication Date: 2006-03-07T01:34:02Z
• Authors: Manoj Pratim Samanta; Waraporn Tongprasit; Himanshu Sethi; Chen-Shan Chin; Viktor Stolc
• Keywords: Tiling array; Small nucleolar RNA

Noncoding RNAs (ncRNAs) perform essential cellular tasks and play key regulatory roles in all organisms. Although several new ncRNAs yeast were recently discovered by individual studies, to our knowledge no comprehensive empirical search has been conducted. We demonstrate a powerful versatile method for global identification of previously undescribed modulating an RNA processing pathway through the depletion ribonucleoprotein enzyme component, monitoring differential transcriptional activities with genome tiling arrays during time course depletion. The entire Saccharomyces cerevisiae was scanned cell growth decay regulated promoter-mediated Rpp1 , functionally conserved protein component RNase P enzyme. In addition most verified genes ncRNAs, expression detected 98 antisense intergenic regions, 74 that further confirmed contain RNAs. A class located coding regions protein-coding genes, is discussed this article. One member, HRA1 likely involved 18S rRNA maturation.