Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls

• DOI: 10.1073/pnas.0604632103
• Publication Date: 2006-07-15T01:55:46Z
• Authors: Stefan Bauer; Prasanna Vasu; Staffan Persson; Andrew J. Mort; Chris R. Somerville
• Keywords: Aspergillus nidulans; Neurospora

To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, fumigatus, Neurospora crassa expressed the as secreted proteins with C-terminal Myc 6x His tags. Most recombinant were active in enzyme assays, optima for pH temperature established. A subset was used to fragment polysaccharides irregular xylem 9 (irx9) mutant Arabidopsis. The revealed a decrease abundance xylan mutant, indicating that IRX9 gene, which encodes putative family 43 glycosyltransferase, is required synthesis.