Hepatitis C virus production by human hepatocytes dependent on assembly and secretion of very low-density lipoproteins

• DOI: 10.1073/pnas.0700760104
• Publication Date: 2007-03-22T00:38:51Z
• Authors: Hua Huang; Fang Sun; David M. Owen; Weiping Li; Yan Chen; Michael Gale; Jin Ye
• Keywords: 0301 basic medicine; Carcinoma, Hepatocellular; Immunomagnetic Separation; Liver Neoplasms; Hepacivirus; Lipoproteins, VLDL; 3. Good health; 03 medical and health sciences; Apolipoproteins E; Cell Line, Tumor; Microsomes; Hepatocytes; Humans; Replicon; RNA, Small Interfering; Triglycerides; Apolipoproteins B

Hepatitis C virus (HCV) and triglyceride-rich very low-density lipoproteins (VLDLs) both are secreted uniquely by hepatocytes circulate in blood a complex. Here, we isolated from human hepatoma cells the membrane vesicles which HCV replicates. These vesicles, contain replication complex, highly enriched proteins required for VLDL assembly, including apolipoprotein B (apoB), apoE, microsomal triglyceride transfer protein. In that constitutively produce infectious HCV, production is reduced two agents block assembly: an inhibitor of protein siRNA directed against apoB. results provide possible explanation restriction to liver suggest new cellular targets treatment infection.