MiR-15a and miR-16-1 cluster functions in human leukemia

• DOI: 10.1073/pnas.0800121105
• Publication Date: 2008-03-25T00:44:45Z
• Authors: George A. Calin; Amelia Cimmino; Muller Fabbri; Manuela Ferracin; Sylwia E. Wojcik; Masayoshi Shimizu; Cristian Taccioli; Nicola Zanesi; Ramiro Garzon; Rami I. Aqeilan; Hansjuerg Alder; Stefano Volinia; Laura Rassenti; Xiuping Liu; Chang-gong Liu; Thomas J. Kipps; Massimo Negrini; Carlo M. Croce
• Keywords: MCL1; Gene signature

MicroRNAs (miRNAs) are short noncoding RNAs regulating gene expression that play roles in human diseases, including cancer. Each miRNA is predicted to regulate hundreds of transcripts, but only few have experimental validation. In chronic lymphocytic leukemia (CLL), the most common adult leukemia, miR-15a and miR-16-1 lost or down-regulated majority cases. After our previous work indicating a tumor suppressor function miR-15a/16-1 by targeting BCL2 oncogene, here, we produced high-throughput profiling genes modulated leukemic cell line model (MEG-01) primary CLL samples. By combining bioinformatics data, identified miR-15a/16-1-gene signature cells. Among components signature, observed statistically significant enrichment AU-rich elements (AREs). examining Gene Ontology (GO) database, cancer (such as MCL1, BCL2, ETS1, JUN) directly indirectly affect apoptosis cycle was found.