Transcriptome-guided characterization of genomic rearrangements in a breast cancer cell line

• DOI: 10.1073/pnas.0812945106
• Publication Date: 2009-01-31T01:55:31Z
• Authors: Qi Zhao; Otavia L. Caballero; Samuel Levy; Brian J. Stevenson; Christian Iseli; Sandro J. de Souza; Pedro A. Galante; Dana Busam; Margaret A. Leversha; Kalyani Chadalavada; Yu-Hui Rogers; J. Craig Venter; Andrew J. G. Simpson; Robert L. Strausberg
• Keywords: Gene Rearrangement; MRE11 Homologue Protein; 0303 health sciences; DNA, Complementary; Base Sequence; Genome, Human; Gene Expression Profiling; Nuclear Proteins; Breast Neoplasms; Histone-Lysine N-Methyltransferase; Neoplasm Proteins; 3. Good health; DNA-Binding Proteins; 03 medical and health sciences; Cell Line, Tumor; Humans; Female; Carrier Proteins

We have identified new genomic alterations in the breast cancer cell line HCC1954, using high-throughput transcriptome sequencing. With 120 Mb of cDNA sequences, we were able to identify rearrangement events leading fusions or truncations genes including MRE11 and NSD1, already implicated oncogenesis, 7 rearrangements involving other additional genes. This approach demonstrates that sequencing is an effective strategy for characterization cancers.