MicroRNA expression profiles predictive of human renal allograft status

• DOI: 10.1073/pnas.0813121106
• Publication Date: 2009-03-17T01:38:29Z
• Authors: Dany Anglicheau; Vijay K. Sharma; Ruchuang Ding; Aurélie Hummel; Catherine Snopkowski; Darshana Dadhania; Surya V. Seshan; Manikkam Suthanth...
• Keywords: Phytohaemagglutinin

Immune rejection of organ transplants is a life-threatening complication and exemplified by alterations in the expression protein-encoding genes. Because microRNAs (miRNAs) regulate genes implicated adaptive immunity, we investigated whether acute (AR) associated with miRNA within allografts profiles are diagnostic AR predict allograft function. Seven 33 renal biopsies (12 21 normal) were profiled using microfluidic cards containing 365 mature human miRNAs (training set), subset differentially expressed quantified remaining 26 (validation set). We found strong association between intragraft messenger RNAs (mRNAs), that AR, function, could be predicted high level precision levels miRNAs. Our investigation normal peripheral blood mononuclear cells (PBMCs) showed (miR-142–5p, -155, -223) overexpressed highly PBMCs, stimulation mitogen phytohaemagglutinin results an increase abundance miR-155 decrease miR-223 let-7c. Quantification primary cultures epithelial (HRECs) miR-30a-3p, -10b, let-7c HRECs, decreased miR-30a-3p. studies, addition to suggesting cellular basis for altered miRNAs, propose patterns may serve as biomarkers status.