Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator

• DOI: 10.1073/pnas.0900592106
• Publication Date: 2009-05-08T00:55:43Z
• Authors: Linqi Wang; Xiuyun Tian; Juan Wang; Haihua Yang; Keqiang Fan; Gangming Xu; Keqian Yang; Huarong Tan
• Keywords: Response regulator; Transcription

In bacteria, many "atypical" response regulators (ARRs) lack the conserved residues important for phosphorylation by which typical switch their output response, suggesting existence of alternative regulatory mechanisms. However, such mechanisms have not been established. JadR1, an OmpR-type ARR Streptomyces venezuelae, appears to activate transcription jadomycin B (JdB) biosynthetic genes while repressing its own gene. JadR1 activities were inhibited in cells induced produce JdB, was found bind directly N-terminal receiver domain causing dissociate from target promoters. The activity a NarL-type ARR, RedZ, that regulates production another antibiotic likewise modulated end product (undecylprodigisines), implying end-product-mediated control pathway-specific ARRs may be widespread. These results could prove relevant knowledge-based improvements yield commercially antibiotics.