ISG56 is a negative-feedback regulator of virus-triggered signaling and cellular antiviral response

• DOI: 10.1073/pnas.0900818106
• Publication Date: 2009-04-29T00:13:01Z
• Authors: Ying Li; Chao Li; Peng Xue; Bo Zhong; Ai-Ping Mao; Yong Ran; He Chen; Yan-Yi Wang; Fuquan Yang; Hong-Bing Shu
• Keywords: Sendai virus; IRF3; Mediator

IFN-stimulated gene 56 (ISG56) is one of the first identified proteins induced by viruses and type I IFNs. In this study, we ISG56 as a virus-induced protein associated with MITA, an adapter involved in virus-triggered induction Overexpression inhibited Sendai activation IRF3, NF-kappaB, IFN-beta promoter, whereas knockdown had opposite effects. Consistently, overexpression reversed cytoplasmic poly(I:C)-induced inhibition vesicular stomatitis virus (VSV) replication, VSV replication. Competitive coimmunoprecipitation experiments indicated that disrupted interactions between MITA VISA or TBK1, two components IFN signaling pathways. These results suggest mediator negative-feedback regulation IFNs cellular antiviral responses.