Rapid detection and profiling of cancer cells in fine-needle aspirates

• DOI: 10.1073/pnas.0902365106
• Publication Date: 2009-07-22T01:07:45Z
• Authors: Hakho Lee; Tae-Jong Yoon; Jose-Luiz Figueiredo; Filip K. Swirski; Ralph Weissleder
• Keywords: Profiling (computer programming); Cancer Detection

There is a growing need for fast, highly sensitive and quantitative technologies to detect profile unaltered cells in biological samples. Technologies current clinical use are often time consuming, expensive, or require considerable sample sizes. Here, we report diagnostic magnetic resonance (DMR) sensor that combines miniaturized NMR probe with targeted nanoparticles detection molecular profiling of cancer cells. The measures the transverse relaxation rate water molecules samples which target interest labeled nanoparticles. We achieved remarkable sensitivity improvements over our prior DMR prototypes by synthesizing new higher relaxivity optimizing assay protocols. detected as few 2 1-μL volumes unprocessed fine-needle aspirates tumors profiled expression several cellular markers <15 min.