The transcriptome of human CD34+hematopoietic stem-progenitor cells

• DOI: 10.1073/pnas.0903390106
• Publication Date: 2009-05-01T01:42:17Z
• Authors: Yeong C. Kim; Qingfa Wu; Jun Chen; Zhenyu Xuan; Yong-Chul Jung; Michael Q. Zhang; Janet D. Rowley; San Ming Wang
• Keywords: Expressed Sequence Tags; Genetic Markers; 0301 basic medicine; 0303 health sciences; DNA, Complementary; Base Sequence; Gene Expression Profiling; Molecular Sequence Data; Antigens, CD34; Hematopoietic Stem Cells; Hematopoiesis; 03 medical and health sciences; Genes; Humans

Studying gene expression at different hematopoietic stages provides insights for understanding the genetic basis of hematopoiesis. We analyzed in human CD34(+) cells that represent stem-progenitor population (CD34(+) cells). collected >459,000 transcript signatures from cells, including de novo-generated 3' ESTs and existing sequences full-length cDNAs, ESTs, serial analysis (SAGE) tags, performed an extensive annotation on this large set sequences. determined genes expressed verified known identified new functional categories involved hematopoiesis, dissected alternative transcription initiation, splicing, adenylation, antisense noncoding transcripts, cell-specific signature, developed cell-transcription map genome. Our study a current view reveals early hematopoiesis is orchestrated process with involvement over half distributed various functions. The data generated our provide comprehensive uniform resource studying stem cell biology.