Quantitative biological imaging by ptychographic x-ray diffraction microscopy

• DOI: 10.1073/pnas.0905846107
• Publication Date: 2009-12-18T05:47:26Z
• Authors: Klaus Giewekemeyer; Pierre Thibault; Sebastian Kalbfle...; André Beerlink; Cameron M. Kewish; Martin Dierolf; Franz Pfeiffer; Tim Salditt
• Keywords: Ptychography; Pinhole (optics); Biological specimen

Recent advances in coherent x-ray diffractive imaging have paved the way to reliable and quantitative of noncompact specimens at nanometer scale. Introduced a year ago, an advanced implementation ptychographic has removed much previous limitations regarding sample preparation illumination conditions. Here, we apply this recent approach toward structure determination nanoscale biological microscopy. We show that projected electron density unstained unsliced freeze-dried cells bacterium Deinococcus radiodurans can be derived from reconstructed phase straightforward reproducible way, with quantified small errors. Thus, may contribute future understanding highly disputed nucleoid bacterial cells. In present study, estimated resolution for was 85 nm (half-period length), whereas 50-nm demonstrated lithographic test structures. With respect diameter pinhole used illuminate samples, superresolution about 15 achieved 30 structures, respectively. These values should assessed view low dose applied on order approximately 1.3x10(5) Gy, were shown scale photon fluence.