WDR5 is essential for assembly of the VISA-associated signaling complex and virus-triggered IRF3 and NF-κB activation

• DOI: 10.1073/pnas.0908967107
• Publication Date: 2009-12-23T02:09:29Z
• Authors: Yan-Yi Wang; Li-Juan Liu; Bo Zhong; Tian-Tian Liu; Ying Li; Yan Yang; Yong Ran; Shu Li; Po Tien; Hong-Bing Shu
• Keywords: IRF3; Transcription

Viral infection causes activation of the transcription factors NF-κB and IRF3, which collaborate to induce type I interferons (IFNs) cellular antiviral response. The mitochondrial outer membrane protein VISA acts as a critical adapter for assembling virus-induced complex that signals IRF3 activation. Using biochemical purification approach, we identified WD repeat WDR5 VISA-associated protein. was recruited in viral dependent manner. also caused translocation from nucleus mitochondria. Knockdown impaired formation complex. Consistently, knockdown inhibited virus-triggered well IFNB1 gene. These findings suggest is essential plays an important role induction IFNs.