PINK1-dependent recruitment of Parkin to mitochondria in mitophagy

• DOI: 10.1073/pnas.0911187107
• Publication Date: 2009-12-06T15:26:48Z
• Authors: Cristofol Vives-B...; Chun Zhou; Yong Huang; Mei Cui; Rosa L.A. de Vries; Jiho Kim; Jessica May; Maja Aleksandra T...; Wencheng Liu; Han Seok Ko; Jordi Magrané; Darren J. Moore; Valina L. Dawson; Regis Grailhe; Ted M. Dawson; Chenjian Li; Kim Tieu; Serge Przedborski
• Keywords: Membrane Potential, Mitochondrial; Carbonyl Cyanide m-Chlorophenyl Hydrazone; 0303 health sciences; Ionophores; Ubiquitin-Protein Ligases; Parkinson Disease; Microtubules; Cell Line; Mitochondria; Protein Transport; 03 medical and health sciences; Oncology; Autophagy; Humans; Biology; Molecular Biology; Protein Kinases; Protein Binding

Phosphatase and tensin homolog (PTEN)-induced putative kinase 1 ( PINK1 ) and PARK2/Parkin mutations cause autosomal recessive forms of Parkinson's disease. Upon a loss of mitochondrial membrane potential (ΔΨ m ) in human cells, cytosolic Parkin has been reported to be recruited to mitochondria, which is followed by a stimulation of mitochondrial autophagy. Here, we show that the relocation of Parkin to mitochondria induced by a collapse of ΔΨ m relies on PINK1 expression and that overexpression of WT but not of mutated PINK1 causes Parkin translocation to mitochondria, even in cells with normal ΔΨ m . We also show that once at the mitochondria, Parkin is in close proximity to PINK1, but we find no evidence that Parkin catalyzes PINK1 ubiquitination or that PINK1 phosphorylates Parkin. However, co-overexpression of Parkin and PINK1 collapses the normal tubular mitochondrial network into mitochondrial aggregates and/or large perinuclear clusters, many of which are surrounded by autophagic vacuoles. Our results suggest that Parkin, together with PINK1, modulates mitochondrial trafficking, especially to the perinuclear region, a subcellular area associated with autophagy. Thus by impairing this process, mutations in either Parkin or PINK1 may alter mitochondrial turnover which, in turn, may cause the accumulation of defective mitochondria and, ultimately, neurodegeneration in Parkinson's disease.