Defined factors induce reprogramming of gastrointestinal cancer cells

• DOI: 10.1073/pnas.0912407107
• Publication Date: 2009-12-15T03:19:27Z
• Authors: Norikatsu Miyoshi; Hideshi Ishii; Ken-ichi Nagai; Hiromitsu Hoshino; Koshi Mimori; Fumiaki Tanaka; Hiroaki Nagano; Mitsugu Sekimoto; Yuichiro Doki; Masaki Mori
• Keywords: Homeobox protein NANOG; Reprogramming; KLF4

Although cancer is a disease with genetic and epigenetic origins, the possible effects of reprogramming by defined factors remain to be fully understood. We studied induction or inhibition cancer-related genes immature status-related whose alterations have been reported in gastrointestinal cells. Retroviral-mediated introduction induced pluripotent stem (iPS) cell was necessary for inducing expression proteins, including Nanog, Ssea4, Tra-1-60, Tra-1-80 esophageal, stomach, colorectal, liver, pancreatic, cholangiocellular Induced cells, but not parental possessed potential express morphological patterns ectoderm, mesoderm, endoderm, which supported studies, indicating methylation DNA strands histone H3 protein at lysine 4 promoter regions pluripotency-associated such as NANOG. In vitro analysis cells showed slow proliferation were sensitized differentiation-inducing treatment, vivo tumorigenesis reduced NOD/SCID mice. This study demonstrated that pluripotency manifested (iPC) distinct from natural regard their sensitivity treatment. iPC confers higher chemotherapeutic agents