Vascular patterning defects associated with expression of activated Notch4 in embryonic endothelium

• DOI: 10.1073/pnas.091584598
• Publication Date: 2002-07-26T14:44:19Z
• Authors: H. Uyttendaele; J. Ho; J. Rossant; J. Kitajewski
• Keywords: Cell fate determination; Hes3 signaling axis

Notch proteins function as receptors for membrane-bound ligands (Jagged and Delta-like) to regulate cell-fate determination. We have investigated the role of signaling in embryonic endothelium mouse by expressing an activated form Notch4 protein vasculature under regulation Flk1 (VEGFR) locus. Expression results a growth developmental delay lethality at about 10 days postcoitum. The extent developing mutant embryos was restricted, fewer small vessels were seen, vascular networks disorganized. brain periphery contained large dilated with evidence compromised vessel-wall integrity areas necrosis; yolk-sac abnormal. leads abnormal vessel structure patterning, implicating pathway phases development associated patterning remodeling.