Real-time multidimensional NMR follows RNA folding with second resolution

• DOI: 10.1073/pnas.1001195107
• Publication Date: 2010-05-04T02:02:58Z
• Authors: Mi-Kyung Lee; Maayan Gal; Lucio Frydman; Gabriele Varani
• Keywords: Riboswitch; Folding (DSP implementation); Nucleic acid structure

Conformational transitions and structural rearrangements are central to the function of many RNAs yet remain poorly understood. We have used ultrafast multidimensional NMR techniques monitor adenine-induced folding an adenine-sensing riboswitch in real time, with nucleotide-resolved resolution. By following changes 2D spectra at rates approximately 0.5 Hz, we identify distinct steps associated ligand-induced riboswitch. Following recognition ligand, long range loop-loop interactions form then progressively stabilized before formation a fully stable complex over 2–3 minutes. The application these methods provides opportunity determine structure RNA intermediates conformational trajectories.