Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays

• DOI: 10.1073/pnas.1001515107
• Publication Date: 2010-08-03T03:35:49Z
• Authors: Antoine-Emmanuel ...; Laure Saias; Eleni Psychari; Nicolas Minc; Damien Simon; François-Clément ...; Claire Mathiot; Jean-Yves Pierga; Vincent Fraisier; Jean Salamero; Véronique Saada; Françoise Farace; Philippe Vielh; Laurent Malaquin; Jean-Louis Viovy
• Keywords: Immunophenotyping; Cytometry

We propose a unique method for cell sorting, “Ephesia,” using columns of biofunctionalized superparamagnetic beads self-assembled in microfluidic channel onto an array magnetic traps prepared by microcontact printing. It combines the advantages notably application well controlled, flow-activated interaction between cells and beads, those immunomagnetic use batch-prepared, characterized antibody-bearing beads. On lines mixtures, we demonstrated capture yield better than 94%, possibility to cultivate situ captured cells. A second series experiments involved clinical samples—blood, pleural effusion, fine needle aspirates— issued from healthy donors patients with B-cell hematological malignant tumors (leukemia lymphoma). The immunophenotype morphology B-lymphocytes were analyzed directly chamber, compared conventional flow cytometry visual cytology data, blind test. Immunophenotyping results Ephesia fully consistent obtained cytometry. high resolution confocal three-dimensional images nuclei, showing intranuclear details cytological staining. thus provides powerful approach typing allowing automated quantitative immunophenotyping morphological analysis. requires at least 10 times smaller sample volume numbers cytometry, potentially increasing range indications success rate microbiopsy-based diagnosis, reducing analysis time cost.