Clostridium ljungdahlii represents a microbial production platform based on syngas

• DOI: 10.1073/pnas.1004716107
• Publication Date: 2010-07-03T03:20:49Z
• Authors: Michael Köpke; Claudia Held; Sandra Hujer; Heiko Liesegang; Arnim Wiezer; Antje Wollherr; Armin Ehrenreich; Wolfgang Liebl; Gerhard Gottschalk; Peter Dürre
• Keywords: Heterologous expression

Clostridium ljungdahlii is an anaerobic homoacetogen, able to ferment sugars, other organic compounds, or CO 2 /H and synthesis gas (CO/H ). The latter feature makes it interesting microbe for the biotech industry, as important bulk chemicals proteins can be produced at expense of , thus combining industrial needs with sustained reduction in atmosphere. Sequencing complete genome C. revealed that comprises 4,630,065 bp one largest clostridial genomes known date. Experimental data silico comparisons a third mode homoacetogenic metabolism. Unlike organisms such Moorella thermoacetica Acetobacterium woodii neither cytochromes nor sodium ions are involved energy generation. Instead, Rnf system present, by which proton translocation performed. An electroporation procedure has been developed transform organism plasmids bearing heterologous genes butanol production. Successful expression these could demonstrated, leading formation biofuel. Thus, used unique microbial production platform based on carbon dioxide/hydrogen mixtures.