Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration

• DOI: 10.1073/pnas.1010795108
• Publication Date: 2010-12-14T04:49:21Z
• Authors: Gloria Assaker; Damien Ramel; Stefanie K. Wculek; Marcos González-G...; Gregory Emery
• Keywords: Exocyst; Cell polarity; Border cells; Small GTPase

Border cell migration is a stereotyped occurring during the development of Drosophila egg chamber. During this process, cluster composed six to eight follicle cells migrates between nurse toward oocyte. Receptor tyrosine kinases (RTKs) are enriched at leading edge and establish directionality their migration. Endocytosis has been shown play role in maintenance polarization; however, mechanisms involved largely unknown. In study, we show that border requires function small GTPases Rab5 Rab11 regulate trafficking through early recycling endosome, respectively. Expression dominant negative form rab11 induces loss polarization RTK activity, which correlates with severe phenotype. addition, demonstrate exocyst component Sec15 distributed structures polarized process Rab11-dependent manner down-regulation different subunits also affects Together, our data fundamental for plasma membrane-endosome cycle active