A chromatin localization screen reveals poly (ADP ribose)-regulated recruitment of the repressive polycomb and NuRD complexes to sites of DNA damage

• DOI: 10.1073/pnas.1012946107
• Publication Date: 2010-10-12T00:53:33Z
• Authors: Danny M. Chou; Britt Adamson; Noah E. Dephoure; Xu Tan; Amanda C. Nottke; Kristen E. Hurov; Steven P. Gygi; Monica P. Colaiácovo; Stephen J. Elledge
• Keywords: HMG-box

Many proteins that respond to DNA damage are recruited lesions. We used a proteomics approach coupled isotopic labeling with chromatin fractionation and mass spectrometry uncover associate damaged DNA, many of which involved in repair or nucleolar function. show polycomb group members by poly(ADP ribose) polymerase (PARP) lesions following UV laser microirradiation. Loss components results IR sensitivity mammalian cells Caenorhabditis elegans . PARP also recruits two the repressive nucleosome remodeling deacetylase (NuRD) complex, chromodomain helicase DNA-binding protein 4 (CHD4) metastasis associated 1 (MTA1), plays role removing nascent RNA elongating II from sites damage. propose sets up transient structure at block transcription facilitate repair.