Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity

• DOI: 10.1073/pnas.1013170108
• Publication Date: 2011-01-25T06:09:22Z
• Authors: Shuangding Li; Robert Esterberg; Veronik Lachance; Dongdong Ren; Kristen Radde-Gal...; Fanglu Chi; Jean-Luc Parent; Andreas Fritz; Ping Chen
• Keywords: Convergent extension; Cell polarity; LRP6; LRP5; PDZ domain

The vertebrate planar cell polarity (PCP) pathway shares molecular components with the β-catenin–mediated canonical Wnt but acts through membrane complexes containing Vang or Frizzled to orient neighboring cells coordinately. interactions underlying action of in PCP signaling and specification, however, are yet be delineated. Here, we report identification Rack1 as an interacting protein a protein, Vangl2. We demonstrate that is required zebrafish for PCP-regulated processes, including oriented division, cellular polarization, convergent extension during gastrulation. further show knockdown affects localization Vangl2 Vangl2-interacting domain has dominant-negative effect on Moreover, antagonizes signaling. Together, our data suggest regulates essential switch promote