Lack of the endosomal SNAREs vti1a and vti1b led to significant impairments in neuronal development

• DOI: 10.1073/pnas.1013891108
• Publication Date: 2011-01-25T06:09:22Z
• Authors: Ajaya J. Kunwar; Michael Rickmann; Bianca Backofen; Sascha M. Browski; Joachim Rosenbusch; Susanne Schöning; Thomas Fleischmann; Kerstin Krieglstein; Gabriele Fischer ...
• Keywords: Axoplasmic transport

Fusion between membranes is mediated by specific SNARE complexes. Here we report that fibroblasts survive the absence of trans-Golgi network/early endosomal vti1a and late vti1b with intact organelle morphology minor trafficking defects. Because are only members their subclass yeast homolog Vti1p essential for cell survival, these data suggest more distantly related SNAREs acquired ability to function in traffic during evolution. However, resulted perinatal lethality. Major axon tracts were missing, reduced size, or misrouted Vti1a −/− Vti1b embryos. Progressive neurodegeneration was observed most peripheral ganglia. Neurons than 95% dorsal root geniculate ganglia at embryonic day 18.5. These special demands membrane could not be met neurons. single deficient mice viable without neuronal defects, indicating they can substitute each other processes.