Golgi nucleotide sugar transporter modulates cell wall biosynthesis and plant growth in rice

• DOI: 10.1073/pnas.1016144108
• Publication Date: 2011-03-08T12:01:21Z
• Authors: Baocai Zhang; Xiangling Liu; Qian Qian; Lifeng Liu; Guojun Dong; Guangyan Xiong; Dali Zeng; Yihua Zhou
• Keywords: Nucleotide sugar; Secondary cell wall

Golgi-localized nucleotide sugar transporters (NSTs) are considered essential for the biosynthesis of wall polysaccharides and glycoproteins based on their characteristic transport a large number sugars to Golgi lumen. The lack NST mutants in plants has prevented evaluation this hypothesis plants. A previously undescribed mutant, brittle culm14 ( bc14 ), displays reduced mechanical strength caused by decreased cellulose content altered structure, exhibits abnormalities plant development. Map-based cloning revealed that all observed mutant phenotypes result from missense mutation putative gene, Oryza sativa Nucleotide Sugar Transporter1 OsNST1 ). was identified as transporter analysis fluorescence-tagged expressed rice protoplast cells demonstration UDP-glucose activity via uptake assays yeast. Compositional analyses total fractionated residues wild-type culms showed deficiency synthesis glucoconjugated , indicating supplies glucosyl substrate formation matrix polysaccharides, thereby modulates biosynthesis. is ubiquitously expressed, with high expression tissues. inferior abnormal development suggest pleiotropic effects cell growth. Identification improved our understanding how polysaccharide regulated NSTs