Human sweet taste receptor mediates acid-induced sweetness of miraculin
Models, Molecular
0301 basic medicine
Protein Conformation
610
neoculin
insights
630
Fluorescence
Cell Line
Receptors, G-Protein-Coupled
taste-modifying protein
Mice
03 medical and health sciences
modifying protein
Animals
Humans
G protein-coupled receptor
umami taste
Glycoproteins
0303 health sciences
molecular-mechanism
Hydrogen-Ion Concentration
Taste Buds
calcium imaging
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
positive allosteric modulator
miracle fruit
gymnemic acid
monkey
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
DOI:
10.1073/pnas.1016644108
Publication Date:
2011-09-27T06:26:14Z
AUTHORS (10)
ABSTRACT
Miraculin (MCL) is a homodimeric protein isolated from the red berries of
Richadella dulcifica
. MCL, although flat in taste at neutral pH, has taste-modifying activity to convert sour stimuli to sweetness. Once MCL is held on the tongue, strong sweetness is sensed over 1 h each time we taste a sour solution. Nevertheless, no molecular mechanism underlying the taste-modifying activity has been clarified. In this study, we succeeded in quantitatively evaluating the acid-induced sweetness of MCL using a cell-based assay system and found that MCL activated hT1R2-hT1R3 pH-dependently as the pH decreased from 6.5 to 4.8, and that the receptor activation occurred every time an acid solution was applied. Although MCL per se is sensory-inactive at pH 6.7 or higher, it suppressed the response of hT1R2-hT1R3 to other sweeteners at neutral pH and enhanced the response at weakly acidic pH. Using human/mouse chimeric receptors and molecular modeling, we revealed that the amino-terminal domain of hT1R2 is required for the response to MCL. Our data suggest that MCL binds hT1R2-hT1R3 as an antagonist at neutral pH and functionally changes into an agonist at acidic pH, and we conclude this may cause its taste-modifying activity.
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