Persistent HIV-1 infection of natural killer cells in patients receiving highly active antiretroviral therapy

• DOI: 10.1073/pnas.102672999
• Publication Date: 2002-07-26T14:46:49Z
• Authors: Antonio Valentin; Margherita Rosati; Daniel J. Patenaude; Angelos Hatzakis; Leondios G. Kostr...; Marios Lazanas; Kathleen M. Wyvill; Robert Yarchoan; George N. Pavlakis
• Keywords: green fluorescent protein; molecular cloning; RNA viruses; CD4-Positive T-Lymphocytes; 0301 basic medicine; Receptors, CXCR4; chemokine receptor CCR5; Receptors, CCR5; Anti-HIV Agents; Human immunodeficiency virus 1; Antigens, CD56; HIV Infections; T lymphocyte activation; Antigens, CD4; 03 medical and health sciences; Human immunodeficiency virus infection; Antiretroviral Therapy, Highly Active; mononuclear cell; Humans; human; virus replication; Human immunodeficiency virus; antiretrovirus agent; human cell; flow cytometry; chemokine receptor CXCR4; article; longitudinal study; natural killer cell; virus load; CD56 Antigen; Lymphocyte Subsets; 3. Good health; persistent virus infection; Killer Cells, Natural; virus expression; priority journal; CD4 Antigens; virus purification; HIV-1

We have identified a subset of CD56 + CD3 − human natural killer (NK) cells that express CD4 and the HIV coreceptors CCR5 CXCR4. These can be productively infected in vitro by both CCR5- CXCR4-using molecular clones HIV-1 CD4-dependent manner. Analysis HIV-infected persons showed viral DNA is present purified NK cells, virus could rescued from these after cultivation. Longitudinal analysis levels patients 1–2 years highly active antiretroviral therapy indicated remain persistently account for substantial amount peripheral blood mononuclear cells. results demonstrate non-T with markers are suggest infection important persistence. The properties reservoir should considered attempts to further optimize therapies.