Global discovery of small RNAs in Yersinia pseudotuberculosis identifies Yersinia -specific small, noncoding RNAs required for virulence

• DOI: 10.1073/pnas.1101655108
• Publication Date: 2011-08-30T02:04:21Z
• Authors: Jovanka T. Koo; Trevis M. Alleyne; Chelsea A. Schiano; Nadereh Jafari; Wyndham W. Lathem
• Keywords: Yersinia pseudotuberculosis; Yersinia pestis; Regulon; Yersinia

A major class of bacterial small, noncoding RNAs (sRNAs) acts by base-pairing with mRNAs to alter the translation from and/or stability transcript. Our laboratory has shown that Hfq, chaperone mediates interaction many sRNAs their targets, is required for virulence enteropathogen Yersinia pseudotuberculosis . This finding suggests play a critical role in regulation this pathogen, but these are not known. Using deep sequencing approach, we identified global set expressed vitro Y. Sequencing RNA libraries bacteria grown at 26 °C and 37 resulted identification 150 unannotated sRNAs. The majority specific, without orthologs either Escherichia coli or Salmonella typhimurium Six specific absent genome closely related species pestis We found expression conserved between differs both timing dependence on suggesting evolutionary changes posttranscriptional species. Deletion multiple leads attenuation pathogen mouse model yersiniosis, as does inactivation conserved, -specific sRNA pneumonic plague. Finally, determined regulon controlled one sRNAs, revealing potential determinants regulated manner.