Early ontogenic origin of the hematopoietic stem cell lineage

• DOI: 10.1073/pnas.1115828109
• Publication Date: 2012-03-06T04:33:43Z
• Authors: Yosuke Tanaka; Misato Hayashi; Yasushi Kubota; Hiroki Nagai; Guojun Sheng; Shin-Ichi Nishikawa; Igor M. Samokhvalov
• Keywords: RUNX1; Hematopoietic stem cell; Lineage (genetic)

Several lines of evidence suggest that the adult hematopoietic system has multiple developmental origins, but ontogenic relationship between nascent populations under this scheme is poorly understood. In an alternative theory, earliest definitive blood precursors arise from a single anatomical location, which constitutes cellular source for subsequent populations. To deconvolute ontogeny, we designed embryo-rescue in key factor Runx1 reactivated Runx1-null conceptuses at specific stages. Using complementary vivo and ex approaches, provide hematopoiesis adult-type stem cells originate predominantly extraembryonic mesoderm. Our data also other sites have been proposed to be sources hierarchy are unlikely play substantial role de novo generation.