Combined linkage and association mapping revealsCYCD5;1as a quantitative trait gene for endoreduplication inArabidopsis

• DOI: 10.1073/pnas.1120811109
• Publication Date: 2012-03-06T04:33:43Z
• Authors: Roel Sterken; Raphaël Kiekens; Joanna Boruc; Fanghong Zhang; Annelies Vercauteren; Ilse Vercauteren; Lien De Smet; Stijn Dhondt; Dirk Inzé; Lieven De Veylder; Eugenia Russinova; Marnik Vuylsteke
• Keywords: Endoreduplication; Candidate gene

Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by differentiation. This alternative cycle results in various levels of endoploidy, reaching 4× or higher one haploid set chromosomes. found animals widespread plants, it plays major role cellular differentiation plant development. Here, we show that variation endoreduplication between Arabidopsis thaliana accessions Columbia-0 Kashmir controlled two quantitative trait loci, ENDO-1 ENDO-2. A local candidate gene association analysis 87 accessions, combined with expression analysis, identified CYCD5;1 as most likely underlying ENDO-2, operating rate-determining factor endoreduplication. In accordance, both overexpression silencing were effective changing DNA ploidy levels, confirming to be previously undescribed .