Cellular transcriptional profiling in influenza A virus-infected lung epithelial cells: The role of the nonstructural NS1 protein in the evasion of the host innate defense and its potential contribution to pandemic influenza

• DOI: 10.1073/pnas.112338099
• Publication Date: 2002-09-30T16:42:53Z
• Authors: Gary K. Geiss; Mirella Salvatore; Terrence M. Tumpey; Victoria S. Carter; Xiuyan Wang; Christopher F. Ba...; Jeffery K. Tauben...; Roger E. Bumgarner; Peter Palese; Michael G. Katze; Adolfo García-Sastre
• Keywords: Viral Pathogenesis

The NS1 protein of influenza A virus contributes to viral pathogenesis, primarily by enabling the disarm host cell type IFN defense system. We examined downstream effects expression during infection on global cellular mRNA levels measuring over 13,000 genes in response with wild-type and mutant viruses human lung epithelial cells. Influenza A/PR/8/34 resulted a significant induction involved pathway. Deletion gene increased number magnitude implicated IFN, NF-κB, other antiviral pathways. Interestingly, different IFN-induced showed sensitivities NS1-mediated inhibition their expression. recombinant C-terminal deletion its induced an intermediate pattern between knockout viruses. Most significantly, containing 1918 pandemic was more efficient at blocking IFN-regulated than parental A/WSN/33 virus. Taken together, our results suggest that cells is significantly influenced sequence gene, demonstrating importance regulating triggered infection.