c-kit + precursors support postinfarction myogenesis in the neonatal, but not adult, heart

• DOI: 10.1073/pnas.1208114109
• Publication Date: 2012-07-31T08:08:52Z
• Authors: Sophy A. Jesty; Michele A. Steffey; Frank K. Lee; Martin Breitbach; Michael Hesse; Shaun Reining; Jane C. Lee; Robert M. Doran; Alexander Yu. Nik...; Bernd K. Fleischmann; Michael I. Kotlikoff
• Keywords: Precursor cell

We examined the myogenic response to infarction in neonatal and adult mice determine role of c-kit + cardiovascular precursor cells (CPC) that are known be present early heart development. Infarction postnatal day 1–3 BAC -EGFP mouse hearts induced localized expansion (c-kit)EGFP within infarct, expression Nkx2.5 mRNA, myogenesis, partial regeneration infarction, with adopting vascular fates. Conversely, resulted a modest induction which did not express or undergo differentiation, but adopted fate indicating lack authentic CPC. Explantation infarcted tissue scid mice, adoptive transfer labeled bone marrow, confirmed cardiac source (neonate) angiogenic (neonate adult) cells. FACS-purified /(αMHC)mCherry − (noncardiac) from microdissected infarcts 6 h underwent forming spontaneously beating myocytes vitro; cre/LoxP mapping identified noncardiac population infarctions, undifferentiated precursors contributes myogenesis. Thus, postinfarct failure is likely due context-dependent restriction following injury does define status.