Scaffolding protein SPIDR/KIAA0146 connects the Bloom syndrome helicase with homologous recombination repair

• DOI: 10.1073/pnas.1220921110
• Publication Date: 2013-03-19T07:06:17Z
• Authors: Li Wan; Jinhua Han; Ting Liu; Shunli Dong; Feng Xie; Hongxia Chen; Jun Huang
• Keywords: Bloom syndrome; Sister chromatid exchange; RecQ helicase

The Bloom syndrome gene product, BLM, is a member of the highly conserved RecQ family. An emerging concept BLM helicase collaborates with homologous recombination (HR) machinery to help avoid undesirable HR events and achieve high degree fidelity during reaction. However, exactly how such coordination occurs in vivo poorly understood. Here, we identified protein termed SPIDR (scaffolding involved DNA repair) as link between machinery. independently interacts RAD51 promotes formation BLM/RAD51-containing complex biological importance. Consistent its role scaffolding for assembly foci, cells depleted show increased rate sister chromatid exchange defects HR. Moreover, depletion leads genome instability causes hypersensitivity damaging agents. We propose that, through providing scaffold cooperation multifunctional DNA-processing complex, not only regulates efficiency HR, but also dictates specific pathway.