Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis

• DOI: 10.1073/pnas.1232420100
• Publication Date: 2003-06-24T17:47:39Z
• Authors: Eilon Woolf; Cuiying Xiao; Ofer Fainaru; Joseph Lotem; Dalia Rosen; Varda Negreanu; Yael Bernstein; Dalia Goldenberg; Ori Brenner; Gideon Berke; Ditsa Levanon; Yoram Groner
• Keywords: RUNX1

The RUNX transcription factors are important regulators of lineage-specific gene expression. bifunctional, acting both as activators and repressors tissue-specific target genes. Recently, we have demonstrated that Runx3 is a neurogenic factor, which regulates development survival proprioceptive neurons in dorsal root ganglia. Here report Runx1 highly expressed thymic medulla cortex, respectively, function CD8 T cells during thymopoiesis. Runx3-deficient (Runx3 KO) mice display abnormalities CD4 expression lineage decisions impairment cell maturation the thymus. A large proportion KO peripheral also CD4, contrast to wild-type, their proliferation ability was largely reduced. In addition, vitro cytotoxic activity alloimmunized peritoneal exudate lymphocytes significantly lower compared with WT mice. compound mutant mouse, null for heterozygous - / ;Runx1 + ), all resulting complete lack single-positive spleen. results provide information on role thymopoiesis suggest act transcriptional decisions.