tRNA tK UUU , tQ UUG , and tE UUC wobble position modifications fine-tune protein translation by promoting ribosome A-site binding

• DOI: 10.1073/pnas.1300781110
• Publication Date: 2013-07-09T02:49:24Z
• Authors: Vanessa Anissa Na...; Kshitiz Tyagi; Namit Ranjan; Andrey L. Konevega; Joerg Mittelstaet; Marina V. Rodnina; Matthias Peter; Patrick G. A. Ped...
• Keywords: Wobble base pair; Eukaryotic translation; Translational efficiency

tRNA modifications are crucial to ensure translation efficiency and fidelity. In eukaryotes, the URM1 ELP pathways increase cellular resistance various stress conditions, such as nutrient starvation oxidative agents, by promoting thiolation methoxycarbonylmethylation, respectively, of wobble uridine cytoplasmic (tK UUU ), (tQ UUG (tE UUC ). Although in vitro experiments have implicated these modulating wobbling capacity efficiency, their exact vivo biological roles remain largely unexplored. Using a combination quantitative proteomics codon-specific reporters, we find that specific gene subset enriched for AAA, CAA, GAA codons is impaired absence - -dependent modifications. Moreover, using native tRNAs demonstrate both enhance binding tK ribosomal A-site. Taken together, our data suggest methoxycarbonylmethylation regulate genes with codon content.