Structure of the archaeal head-tailed virus HSTV-1 completes the HK97 fold story

• DOI: 10.1073/pnas.1303047110
• Publication Date: 2013-06-04T05:42:51Z
• Authors: Maija K. Pietilä; Pasi Laurinmäki; Daniel A. Russell; Ching-Chung Ko; Deborah Jacobs-Sera; Roger W. Hendrix; Dennis H. Bamford; Sarah J. Butcher
• Keywords: Archaeal Viruses; Models, Molecular; 0301 basic medicine; Protein Folding; Haloarcula; Cryoelectron Microscopy; Molecular Sequence Data; Genome, Viral; 3. Good health; 03 medical and health sciences; Capsid; Imaging, Three-Dimensional; Capsid Proteins; Computer Simulation

It has been proposed that viruses can be divided into a small number of structure-based viral lineages. One these lineages is exemplified by bacterial virus Hong Kong 97 (HK97), which represents the head-tailed dsDNA bacteriophages. Seemingly similar also infect archaea. Here we demonstrate using genomic analysis, electron cryomicroscopy, and image reconstruction major coat protein fold newly isolated archaeal Haloarcula sinaiiensis tailed 1 canonical HK97. Although it anticipated previously, this physical evidence share common architectural principle. The HK97-like previously recognized in herpesviruses, study expands lineage to from all three domains life. This only second established include archaeal, bacterial, eukaryotic viruses. Thus, our findings support hypothesis last universal ancestor cellular organisms was infected different