Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light

• DOI: 10.1073/pnas.1311632110
• Publication Date: 2013-08-06T05:36:09Z
• Authors: Hou-Sung Jung; Peter A. Crisp; Gonzalo M. Estavillo; Benjamin Cole; Fangxin Hong; Todd C. Mockler; Barry J. Pogson; Joanne Chory
• Keywords: Heat shock factor; Light intensity

Sunlight provides energy for photosynthesis and is essential nearly all life on earth. However, too much or little light rapidly fluctuating conditions cause stress to plants. Rapid changes in the amount of are perceived as a change reduced/oxidized (redox) state photosynthetic electron transport components chloroplasts. how this generates signal that relayed nuclear gene expression not well understood. We modified redox reference plant, Arabidopsis thaliana, using either excess low plus herbicide DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone), well-known inhibitor transport. Modification caused common set about 750 genes, many which known stress-responsive genes. Among most highly enriched promoter elements induced were heat-shock (HSEs), motifs response high temperature systems. show HSEs from ASCORBATE PEROXIDASE 2 (APX2) necessary sufficient APX2 light, under herbicide. tested phenotypes overexpression loss-of-function mutants 15 A-type transcription factors (HSFs), identified HSFA1D, HSFA2, HSFA3 key regulating diverse conditions. Excess regulates both subcellular location HSFA1D its biochemical properties, making it early component network