Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series epitopes

• DOI: 10.1073/pnas.1312457110
• Publication Date: 2013-08-02T05:07:27Z
• Authors: Shih-Fen Liao; Chi-Hui Liang; Ming-Yi Ho; Tsui-Ling Hsu; Tsung-I Tsai; Yves S.-Y. Hsieh; Chih-Ming Tsai; Shiou-Ting Li; Yang-Yu Cheng; Shu-Ming Tsao; Tung-Yi Lin; Zong-Yan Lin; Wen-Bin Yang; Chien-Tai Ren; Kuo-I Lin; Kay-Hooi Khoo; Chun-Hung Lin; Hsien-Yeh Hsu; Chung-Yi Wu; Chi-Huey Wong
• Keywords: Glycobiology

Carbohydrate-based vaccines have shown therapeutic efficacy for infectious disease and cancer. The mushroom Ganoderma lucidum (Reishi) containing complex polysaccharides has been used as antitumor supplement, but the mechanism of immune response rarely studied. Here, we show that mice immunized with a l -fucose (Fuc)-enriched Reishi polysaccharide fraction (designated FMS) induce antibodies against murine Lewis lung carcinoma cells, increased antibody-mediated cytotoxicity reduced production tumor-associated inflammatory mediators (in particular, monocyte chemoattractant protein-1). showed significant increase in peritoneal B1 B-cell population, suggesting FMS-mediated anti-glycan IgM production. Furthermore, glycan microarray analysis FMS-induced antisera displayed high specificity toward glycans, antigenic structure located nonreducing termini (i.e., Fucα1-2Galβ1-3GalNAc-R, where Gal, GalNAc, R represent, respectively, D-galactose, D-N-acetyl galactosamine, reducing end), typically found Globo H related tumor antigens. composition FMS contains mainly backbone 1,4-mannan 1,6-α-galactan through Fucα1-2Gal, Fucα1-3/4Man, Fucα1-4Xyl, Fucα1-2Fuc linkages (where Man Xyl represent d -mannose -xylose, respectively), underlying molecular basis tumor-specific glycans.