Superpriming of synaptic vesicles after their recruitment to the readily releasable pool

• DOI: 10.1073/pnas.1314427110
• Publication Date: 2013-08-27T02:24:39Z
• Authors: Jae Sung Lee; Won-Kyung Ho; Erwin Neher; Suk-Ho Lee
• Keywords: 0301 basic medicine; 0303 health sciences; Phosphodiesterase Inhibitors; Models, Neurological; Excitatory Postsynaptic Potentials; Nerve Tissue Proteins; Actins; Pyrrolidinones; Rats; Diglycerides; Rats, Sprague-Dawley; Kinetics; 03 medical and health sciences; Calmodulin; Type C Phospholipases; Animals; Calcium Signaling; Synaptic Vesicles; Estrenes; Brain Stem

Significance During sustained nerve activity, synapses must continuously recycle vesicles. We used the unique opportunities for quantitative analysis offered by the calyx of Held synapse to study late stages in the process that renders vesicles release-ready. We dissect two sequential steps with distinct pharmacology and kinetics, the characterization of which is essential for an understanding of molecular mechanisms of transmitter release and short-term plasticity.