In vitro evolution of α-hemolysin using a liposome display

• DOI: 10.1073/pnas.1314585110
• Publication Date: 2013-10-01T06:09:28Z
• Authors: Satoshi Fujii; Tomoaki Matsuura; Takeshi Sunami; Yasuaki Kazuta; Tetsuya Yomo
• Keywords: Hemolysin

Significance The directed evolution of proteins in vitro has generated highly functional and contributed to elucidating the sequence–function relationship proteins. However, available methods consider globular proteins, not membrane despite biological pharmaceutical importance latter. We report development a method called liposome display, which enables applied evolve pore-forming activity α-hemolysin from Staphylococcus aureus obtained mutant with 30-fold higher than WT. Given its high degree controllability, display allows for rapid efficient wide range thereby improving field protein engineering.