Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation

• DOI: 10.1073/pnas.1318547111
• Publication Date: 2013-12-17T02:44:35Z
• Authors: Hidetaka Kioka; Hisakazu Kato; Makoto Fujikawa; Osamu Tsukamoto; Toshiharu Suzuki; Hiromi Imamura; Atsushi Nakano; Shuichiro Higo; Satoru Yamazaki; Takashi Matsuzaki; Kazuaki Takafuji; Hiroshi Asanuma; Masanori Asakura; Tetsuo Minamino; Yasunori Shintani; Masasuke Yoshida; Hiroyuki Noji; Masafumi Kitakaze; Issei Komuro; Yoshihiro Asano; Seiji Takashima
• Keywords: 0301 basic medicine; Microscopy, Confocal; Cell Survival; G1 Phase; Cell Cycle Proteins; Biosensing Techniques; Oxidative Phosphorylation; Mitochondria; 3. Good health; Mice; 03 medical and health sciences; Adenosine Triphosphate; HEK293 Cells; Oxygen Consumption; Animals; Humans; Cattle; Myocytes, Cardiac; Oligomycins; Phosphorylation; Genes, Switch; HeLa Cells; Oligonucleotide Array Sequence Analysis

Significance

We developed a sensitive method to assess the activity of oxidative phosphorylation in living cells using a FRET-based ATP biosensor. We then revealed that G0/G1 switch gene 2, a protein rapidly induced by hypoxia, increases mitochondrial ATP production by interacting with F o F 1 -ATP synthase and protects cells from a critical energy crisis.