Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes

• DOI: 10.1073/pnas.1322727111
• Publication Date: 2014-04-01T06:13:03Z
• Authors: Peyton Shieh; M. Sloan Siegrist; Andrew J. Cullen; Carolyn R. Bertozzi
• Keywords: Bioorthogonal Chemistry; Biomolecule

Significance Bioorthogonal chemistry has created new opportunities for interrogating biomolecules in cells and organisms. Near-infrared (NIR) fluorogenic probes activated by bioorthogonal are ideal imaging studies, as tissue penetrance is highest background fluorescence from excess probe endogenous minimized at these wavelengths. Here, we present the development optimization of NIR azide that undergo a enhancement up to 48-fold upon reaction with terminal or strained alkynes. In conjunction class cyclooctyne d -amino acids, were used image bacterial peptidoglycan (PG) without need cytotoxic copper catalyst washing away probe. This platform should facilitate vivo PG pathogenic bacteria chemistry.