Enhanced microtubule-dependent trafficking and p53 nuclear accumulation by suppression of microtubule dynamics

• DOI: 10.1073/pnas.132275599
• Publication Date: 2002-09-30T16:42:53Z
• Authors: Paraskevi Giannak...; Michel Nakano; Kyriacos C. Nicolaou; Aurora O'Brate; Jian Yu; Mikhail V. Blagos...; Urs F. Greber; Tito Fojo
• Keywords: Puma

The tumor suppressor protein p53 localizes to microtubules (MT) and, in response DNA damage, is transported the nucleus via MT minus-end-directed motor dynein. Dynein also responsible for MT-mediated nuclear targeting of adenovirus type 2 (Ad2). Here we show that treatment with low concentrations MT-targeting compounds (MTCs) do not disrupt network but are known suppress dynamics enhanced accumulation, and activation p53-downstream target genes. accumulation required binding MTCs MTs induction p53-up-regulated modulator apoptosis (PUMA) mRNA on challenging cells DNA-damaging drug adriamycin. Low rate movement fluorescent Ad2 increased efficiency Ad2. We propose suppression by enhances MT-dependent trafficking toward minus ends facilitates targeting.