Mechanism for neutralizing activity by the anti-CMV gH/gL monoclonal antibody MSL-109

• DOI: 10.1073/pnas.1404653111
• Publication Date: 2014-05-20T02:36:03Z
• Authors: Ashley E. Fouts; Laëtitia Comps-Agrar; Katharina F. Stengel; Diego Ellerman; Allyn J. Schoeffler; Søren Warming; Dan L. Eaton; Becket Feierbach
• Keywords: Infectivity; Cytomegalovirus

Significance The CMV glycoprotein (g) entry complex gH/gL is a significant target of the human immune system, eliciting production highly neutralizing antibodies. MSL-109, monoclonal antibody that binds to gH, potently neutralizes entry. Here, viral mutants resistant MSL-109 were generated and epitope mapped on model structure gH. We have used cell-surface fluorescence assay reveal novel interactions among glycoproteins probe effect interactions. Our data confirm cross-talk between gB, fusion protein. Moreover, we identify homophilic interaction heterodimers perturbed by MSL-109. This study suggests mechanism neutralization, highlights potential strategies for treatment disease.