Neurite outgrowth induced by NGF or L1CAM via activation of the TrkA receptor is sustained also by the exocytosis of enlargeosomes

• DOI: 10.1073/pnas.1406097111
• Publication Date: 2014-11-11T04:42:34Z
• Authors: Federico Colombo; Gabriella Racchetti; Jacopo Meldolesi
• Keywords: Neurite; Munc-18

Significance Much of the available information on NGF-induced neural cell differentiation has been first obtained by study a cultured line, pheochromocytoma PC12. Using PC12 cells, we now demonstrate two new aspects process, possibly important also in neurons: ( i ) direct binding, activation, and signaling NGF receptor TrkA adhesion protein, L1CAM, when administered as soluble construct surface-exposed adjacent cells; ii expression, exposed for few hours to treatment, an exocytic vesicle, enlargeosome, that cooperates with endocytic vesicle enlargement surface, which is necessary neurite outgrowth take place.