Improving fatty acids production by engineering dynamic pathway regulation and metabolic control

• DOI: 10.1073/pnas.1406401111
• Publication Date: 2014-07-22T00:59:36Z
• Authors: Peng Xu; Lingyun Li; Fuming Zhang; Gregory Stephanop...; Mattheos Koffas
• Keywords: Metabolic Engineering; Metabolic pathway; Synthetic Biology

Significance One important synthetic chemistry reaction endowed by nature is the decarboxylative carbon condensation using malonyl-CoA as donor. Previous metabolic engineering efforts centered on malonyl-CoA–dependent pathway have resulted in production of many value-added compounds. Here we mimicked native biological systems and used a dynamic regulatory network to optimize titers yield. The naturally existing transcriptional regulator FapR was rewired dynamically control gene expressions involved supply consumption malonyl-CoA. Applying this allowed engineered cell regulate expression compensated activity critical enzymes. switch study opens up new venues for optimization efficient malonyl-CoA–derived