Systematic identification of signal integration by protein kinase A

• DOI: 10.1073/pnas.1409938112
• Publication Date: 2015-03-24T03:17:43Z
• Authors: Marie Filteau; Guillaume Diss; Francisco Torres-...; Alexandre K. Dubé; Andrea Schraffl; Verena A. Bachmann; Isabelle Gagnon-A...; Andrée-Ève Chrétien; Anne-Lise Steunou; Ugo Dionne; Jacques Côté; Nicolas Bisson; Eduard Stefan; Christian R. Landry
• Keywords: Identification; SIGNAL (programming language)

Cellular processes and homeostasis control in eukaryotic cells is achieved by the action of regulatory proteins such as protein kinase A (PKA). Although outbound signals from PKA directed to metabolism, growth, aging have been well charted, what regulates this conserved regulator remains be systematically identified understand how it coordinates biological processes. Using a yeast reporter assay, we genes that influence activity measuring protein-protein interactions between two catalytic subunits complex 3,726 genetic-deletion backgrounds grown on carbon sources. Overall, nearly 500 were found connected directly or indirectly regulation, including 80 core regulators, denoting wide diversity regulating PKA, within beyond described upstream linear pathways. regulators span multiple processes, antagonistic autophagy methionine biosynthesis Our results converge toward mechanisms posttranslational regulation lysine acetylation, which humans that, show, formation mammals carbohydrate storage yeast. Taken together, these show extent input matches with its output, because receives information downstream highlight are interconnected coordinated PKA.