Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea

• DOI: 10.1073/pnas.1419513111
• Publication Date: 2014-11-20T08:43:24Z
• Authors: Lukas Chavez; Yun Huang; Khai Luong; Suneet Agarwal; Lakshminarayan M....; William A. Pastor; Virginia K. Hench; Sylvia A. Frazier...; Evgenia Korol; Shuo Liu; Mamta Tahiliani; Yinsheng Wang; Tyson A. Clark; Jonas Korlach; Patricia J. Pukkila; L. Aravind; Anjana Rao
• Keywords: 0303 health sciences; Basidiomycota; Hyphae; Gene Expression Regulation, Developmental; DNA Methylation; Spores, Fungal; Dioxygenases; Fungal Proteins; 03 medical and health sciences; HEK293 Cells; Gene Expression Regulation, Fungal; 5-Methylcytosine; DNA Transposable Elements; Humans; Chromosomes, Fungal; Genome, Fungal; Oxidation-Reduction; Sequence Analysis

Significance A prominent epigenetic mechanism for gene regulation is methylation of cytosine bases in DNA. TET enzymes facilitate DNA demethylation by converting 5-methylcytosine (5mC) to oxidized methylcytosines (oxi-mCs). We show that oxi-mCs are generated conserved TET/JBP encoded the genome model organism Coprinopsis cinerea and present a method simultaneous mapping three different species at near–base-pair resolution. observe centromeres transposable elements exhibit distinctive patterns 5mC oxi-mC, body oxi-mC mark silent paralogous multicopy genes. Our study describes map simultaneously reveals colocation functional throughout C. genome.