Inhibition of tumor cell proliferation and motility by fibroblasts is both contact and soluble factor dependent

0301 basic medicine Contact Inhibition Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Fibroblasts Coculture Techniques Cell Line Extracellular Matrix 3. Good health Luminescent Proteins Mice 03 medical and health sciences Microscopy, Fluorescence Cell Movement Cell Line, Tumor Culture Media, Conditioned Animals Humans Cells, Cultured Cell Proliferation Oligonucleotide Array Sequence Analysis Red Fluorescent Protein
DOI: 10.1073/pnas.1419554111 Publication Date: 2014-11-20T08:43:24Z
ABSTRACT
Significance Normal microenvironments can restrict cancer development and progression. Inhibition of tumor cell growth and motility by normal fibroblasts is one measurable manifestation of this multicomponential control. Here we show that inhibition withstands formalin fixation and can be augmented by the addition of conditioned medium derived from live cultures of tumor cells confronting the stromal fibroblasts. We describe a number of molecules involved in this process. This study lays the foundation for further mechanistic studies of this important phenomenon and its contribution to possible dormancy and the tumor’s resistance to therapy.
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