Inhibition of tumor cell proliferation and motility by fibroblasts is both contact and soluble factor dependent

• DOI: 10.1073/pnas.1419554111
• Publication Date: 2014-11-20T08:43:24Z
• Authors: Twana Alkasalias; Emilie Flaberg; Vladimir Kashuba; Andrey Alexeyenko; Tatiana Pavlova; Andrii Savchenko; Laszlo Szekely; George Klein; Hayrettin Guven
• Keywords: 0301 basic medicine; Contact Inhibition; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Profiling; Fibroblasts; Coculture Techniques; Cell Line; Extracellular Matrix; Luminescent Proteins; Mice; 03 medical and health sciences; Microscopy, Fluorescence; Cell Movement; Cell Line, Tumor; Culture Media, Conditioned; Animals; Humans; Cells, Cultured; Cell Proliferation; Oligonucleotide Array Sequence Analysis; Red Fluorescent Protein

Significance Normal microenvironments can restrict cancer development and progression. Inhibition of tumor cell growth and motility by normal fibroblasts is one measurable manifestation of this multicomponential control. Here we show that inhibition withstands formalin fixation and can be augmented by the addition of conditioned medium derived from live cultures of tumor cells confronting the stromal fibroblasts. We describe a number of molecules involved in this process. This study lays the foundation for further mechanistic studies of this important phenomenon and its contribution to possible dormancy and the tumor’s resistance to therapy.