Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum

0301 basic medicine Cystic Fibrosis Riboflavin Stem Cells Sputum Biotin Polymerase Chain Reaction Pantothenic Acid 03 medical and health sciences Species Specificity Pseudomonas aeruginosa Humans Wounds and Injuries Computer Simulation Pseudomonas Infections Lung Monte Carlo Method Genome, Bacterial
DOI: 10.1073/pnas.1419677112 Publication Date: 2015-03-17T02:56:33Z
ABSTRACT
Significance The opportunistic pathogen Pseudomonas aeruginosa thrives in cystic fibrosis (CF) lung sputum. Here, we define the essential genome of two P. aeruginosa strains in laboratory media and in CF sputum. We also use genomic methods to profile P. aeruginosa genetic requirements for fitness in both natural and synthetic CF sputum. Finally, we show that the essential genomes of different strains of P. aeruginosa are distinct, suggesting that the architecture of genetic networks is a primary determinant of a gene’s role in fitness. This has implications for the development of strain-independent therapeutics and underscores the importance of functional studies in pathogenic strains of interest.
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