Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum
0301 basic medicine
Cystic Fibrosis
Riboflavin
Stem Cells
Sputum
Biotin
Polymerase Chain Reaction
Pantothenic Acid
03 medical and health sciences
Species Specificity
Pseudomonas aeruginosa
Humans
Wounds and Injuries
Computer Simulation
Pseudomonas Infections
Lung
Monte Carlo Method
Genome, Bacterial
DOI:
10.1073/pnas.1419677112
Publication Date:
2015-03-17T02:56:33Z
AUTHORS (5)
ABSTRACT
Significance
The opportunistic pathogen
Pseudomonas aeruginosa
thrives in cystic fibrosis (CF) lung sputum. Here, we define the essential genome of two
P. aeruginosa
strains in laboratory media and in CF sputum. We also use genomic methods to profile
P. aeruginosa
genetic requirements for fitness in both natural and synthetic CF sputum. Finally, we show that the essential genomes of different strains of
P. aeruginosa
are distinct, suggesting that the architecture of genetic networks is a primary determinant of a gene’s role in fitness. This has implications for the development of strain-independent therapeutics and underscores the importance of functional studies in pathogenic strains of interest.
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CITATIONS (390)
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