Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair

• DOI: 10.1073/pnas.1420115112
• Publication Date: 2015-05-05T02:02:32Z
• Authors: Dylan A. Reid; Sarah Keegan; Alejandra Leo-Macias; Go Watanabe; Natasha T. Strande; Howard H. Chang; Betul Akgol Oksuz; David Fenyo; Michael R. Lieber; Dale A. Ramsden; Eli Rothenberg
• Keywords: Non-homologous end joining; Homology directed repair; Double strand

Significance Nonhomologous end-joining (NHEJ) is the main pathway for repair of DNA double-strand breaks (DSBs), most cytotoxic form damage resulting from ionizing radiation, chemotherapeutics, and normal cellular processes. The mechanisms that control NHEJ play key roles in development, immunity, response to cancer therapy; however, current state knowledge regarding physical nature process limited. Here we used super-resolution microscopy define organization complexes cells, showing long filaments at either side break. Single-molecule FRET revealed dynamic behavior which can pair an adjacent, non–end-to-end configuration.